Release

ProPhylER 1.0 is live now.

News

January 5 2010
The ProPhylER paper is now published in Genome Research

March 12 2010
Searching by name is now supported on the search page

March 12 2010
Searching with hg 18 coordinates for evaluating coding SNPs is now supported

Contacts

prophyler [at] prophyler.org
arend [at] stanford.edu

Resource Links

Ensembl
Uniprot
PDB
WuBlast
Probcons
Semphy
Jmol
Java

Other Links

Sidow Lab
Stanford Pathology Dept
Stanford Genetics Dept
Stanford School of Medicine

Funded by

NIH/NHGRI

Ensembl Identifiers in ProPhylER

You can use either gene or protein identifiers. What's the difference? One gene may give rise to several proteins by alternative splicing, so for one gene identifier there may be several protein identifiers, one per splice isoform. For example, human Jagged2/Serrate2 is gene ENSG00000184916, which encodes proteins ENSP00000328169 and ENSP00000328566.

Be aware that ProPhylER currently uses only one splice isoform in its alignments and analyses. You may find that ProPhylER uses a different isoform from the one that you specified in your search.

Examples of Ensembl IDs by Species

Species Gene ID Protein ID
Homo sapiens (human) ENSG00000131462 ENSP00000251413
Mus musculus (mouse) ENSMUSG00000035198 ENSMUSP00000048036
Gallus gallus (chick) ENSGALG00000003150 ENSGALP00000004979
Danio rerio (zebrafish) ENSDARG00000015610 ENSDARP00000016775
Ciona intestinalis ENSCING00000014985 ENSCINP00000014574
Drosophila melanogaster CG3157 CG3157-PA
Anopheles gambiae ENSANGG00000016077 ENSANGP00000018566
Caenorhabditis elegans F58A4.8 F58A4.8.1
Saccharomyces cerevisiae TUB4 YLR212C

You need to input the entire identifier exactly to get a match in ProPhylER.

Uniprot Identifiers in ProPhylER

Below are a few examples of Uniprot IDs. Note that they are all six characters long, with a mix of letters and numbers and starting with a letter.

Q5XLE5
P02114
Q52MT0
Q6J1Z7
Q9BWV6

PDB Identifiers

Below are a few examples of PDB IDs. They are four characters long and start with a digit.

2IAE
2P8Q
2BH9
2RK7
2PGF
2DMW

ProPhylER Cluster Identifiers

are just numbers.

Chromosome Coordinates (Human only, assembly hg 18 only)

Only those coordinates that correspond to a nucleotide in a codon will return results. Noncoding coordinates will not return results. The results do not inform about whether your SNP changes an amino acid, or which amino acid is changed. ProPhylER's results are agnostic to the state of human polymorphisms, reporting MAPP values of all possible amino acids, not just those that are accessible by a single nucleotide change in the position that you queried with.

Here are some Chr:Coordinate; values to test.

6:509145;
10:1050218;
10:1058639;
17:594936;
Y:22039841;
X:63362184;
X:153347528;
X:153594128;


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Last updated 1/25/10